Fig. 6. Posttranscriptional modulation of V5-αENaC deletion mutants mRNA in cells that overexpress Dhx36 and Tial1. Alveolar epithelial cells were cotransfected with the different 3'UTR deletion mutants (Del 1-4) in the pTRE-tight vector along with pTet-Off plasmid and the expression vector for Dhx36 or Tial1 RBP overexpression. V5-αENaC mRNA expression was quantified by RT-qPCR 72 h posttransfection and expressed as percentage of V5-αENaC mRNA compared to cells transfected with an empty vector (pcDNA3) ± SEM after normalization with tTA-Ad. Comparison to the complete 3'UTR in shown in Fig. S6. Overexpression of Dhx36 and Tial1 significantly inhibited V5-αENaC mRNA expression for each construction except for V5-αENaC-Del4, which lacks the 3'UTR sequences. *P<0.05 by the Kruskal-Wallis tests and Dunn's post-hoc tests compared to empty vector; #P<0.05 by the Mann-Whitney U-tests compared to complete 3'UTR mutant; n≥6 for each experimental condition.